| Field | Specification |
|---|---|
| Target | |
| Alternative names | RNF146, Dactylidin, RING finger protein 146, E3 ubiquitin-protein ligase RNF146, RING-type E3 ubiquitin transferase RNF146, Iduna |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Applications | |
| Purity | |
| Purification | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Antibody against Iduna/RNF146 (E3 ubiquitin-protein ligase RNF146) for research detection of protein expression and localization.
Target biology
E3 ubiquitin-protein ligase RNF146 is a ligase that catalyzes formation of covalent bonds (often ATP-dependent) and can participate in processes such as ubiquitin-like conjugation, DNA repair or metabolic pathway assembly depending on the ligase family. Ligases are commonly investigated for roles in protein turnover and cellular stress responses.
Expected localization
Localization varies by ligase family; many are cytosolic/nuclear, with some organellar forms.
Research context
This target is frequently studied in the context of Protein Homeostasis & Degradation research. Application suitability (e.g., IHC, WB) is indicated in the specifications; conditions should be optimized by titration.
Recommended experimental notes
- For Western blotting, optimize lysis conditions and include reducing/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.
- For IHC, fixation and antigen retrieval strongly influence epitope accessibility; test citrate- vs EDTA-based retrieval and include matched positive/negative tissues.
Controls & interpretation
Where possible, confirm specificity using genetic perturbation (KO/KD/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low/none of Iduna/RNF146, and a positive control sample known to express Iduna/RNF146.
For research use only. Not for diagnostic procedures.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
