| Field | Specification |
|---|---|
| Target | |
| Alternative names | Gamma-interferon-inducible lysosomal thiol reductase; Gamma-interferon-inducible protein IP-30; Legumaturain; IFI30; GILT; IP30 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human IFI30 recombinant protein (Position: S27-K250). Human IFI30 shares 66.3% and 68.8% amino acid (aa) sequence identity with mouse and rat IFI30, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects gamma-interferon-inducible lysosomal thiol reductase (gene IFI30) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
IFI30 (also called GILT) is a lysosomal thiol reductase that is expressed constitutively in antigen-presenting cells and induced by interferon-γ. It reduces disulfide bonds in endocytosed proteins, helping them unfold for efficient processing and presentation on MHC class II and, in some cases, MHC class I molecules.
| Target | Gamma-interferon-inducible lysosomal thiol reductase (gene IFI30; UniProt P13284, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human IFI30 fragment (Ser27–Lys250), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 66.3% and 68.8%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes gamma-interferon-inducible lysosomal thiol reductase as secreted and also located in the lysosome. Tissue expression noted by UniProt (human): Expressed constitutively in antigen-presenting cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
