| Field | Specification |
|---|---|
| Target | |
| Alternative names | IFIT2; CIG-42; G10P2; IFI54; ISG54; Interferon-induced protein with tetratricopeptide repeats 2; IFIT-2; ISG-54 K; Interferon-induced 54 kDa protein; IFI-54K; P54 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human IFIT2 recombinant protein (Position: N23-E458). Human IFIT2 shares 62.2% amino acid (aa) sequence identity with mouse IFIT2. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets interferon-induced protein with tetratricopeptide repeats 2 (gene IFIT2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA.
IFIT2 (ISG54) is an interferon-induced protein with tetratricopeptide repeats that restricts virus replication. It binds viral RNAs, including those lacking the 2′-O-methylated cap found on host mRNAs, and interferes with their translation; it can also promote apoptosis, and it is studied in innate antiviral responses.
| Target | Interferon-induced protein with tetratricopeptide repeats 2 (gene IFIT2; UniProt P09913, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human IFIT2 fragment (Asn23–Glu458), expressed in E. coli. Sequence identity with the mouse ortholog: 62.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places interferon-induced protein with tetratricopeptide repeats 2 in the cytoplasm and endoplasmic reticulum.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
