| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon gamma;IFN-gamma;Immune interferon;IFNG; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human IFN gamma recombinant protein (Position: Q24-Q166). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes interferon gamma (gene IFNG) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 19.3 kDa, and the supplier reports an observed band at about 17 kDa.
Interferon gamma is the only type II interferon. It is produced mainly by activated T cells and NK cells, signals through IFNGR1/IFNGR2 and the JAK1/JAK2–STAT1 pathway, and activates macrophages and antigen presentation, making it a key readout of Th1 and cytotoxic immune responses.
| Target | Interferon gamma (gene IFNG; UniProt P01579, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 19.3 kDa |
| Observed MW | 17 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human IFN gamma fragment (Gln24–Gln166), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes interferon gamma as a secreted protein. Tissue expression noted by UniProt (human): Released primarily from activated T lymphocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls. A carrier-free (BSA-free) format can be requested; specify it in the order note.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shanshan Kuang et al. (2026) Indoleamine 2,3-dioxygenase 1 mediated macrophage oxidative phosphorylation impairment drives pro-inflammatory M1 polarization aggravates acetaminophen-induced acute liver injury. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2026.119774
Wang Yadong et al. (2017) NKG2D modulates aggravation of liver inflammation by activating NK cells in HBV infection. Scientific Reports. 10.1038/s41598-017-00221-9
Wang Yadong et al. (2017) Predictive Value of Serum IFN-γ inducible Protein-10 and IFN-γ/IL-4 Ratio for Liver Fibrosis Progression in CHB Patients. Scientific Reports. 10.1038/srep40404
Li-qin Wang et al. (2016) Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1α-Hydroxylase by the Fetal-Maternal Interface. PLoS One. 10.1371/journal.pone.0165589
Zhong Ying-Qiang et al. (2011) Expression of IFN-γ and IL-4 in the Esophageal Mucosa of Patients with Reflux Esophagitis and Barrett’s Esophagus and Their Relationship with Endoscopic and Histologic Grading. Digestive Diseases and Sciences. 10.1007/s10620-011-1696-9
