| Field | Specification |
|---|---|
| Target | |
| Alternative names | Intraflagellar transport protein 172 homolog; IFT172; KIAA1179 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human IFT172 recombinant protein (Position: D1505-Q1736). Human IFT172 shares 94.8% amino acid (aa) sequence identity with both mouse and rat IFT172. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes intraflagellar transport protein 172 homolog (gene IFT172) in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 197.6 kDa, and the supplier reports an observed band at about 180 kDa.
IFT172 is a large component of intraflagellar transport complex B, the machinery that carries cargo along cilia. It is needed to build and maintain cilia and is involved in the turnaround of IFT trains at the ciliary tip, and because Hedgehog signaling depends on primary cilia, it is also studied in ciliopathies and developmental signaling.
| Target | Intraflagellar transport protein 172 homolog (gene IFT172; UniProt Q9UG01, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 197.6 kDa |
| Observed MW | 180 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells, human HEL cells, human U251 cells, human 293T cells, rat testis tissue, rat brain tissue, mouse testis tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human IFT172 fragment (Asp1505–Gln1736), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 94.8%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places intraflagellar transport protein 172 homolog in the cell projection. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
