Anti-IGF1/Igf I Rabbit Monoclonal Antibody

SKU:BHA21007817
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-IGF1 antibody (Rabbit monoclonal, clone DBG-9, Rabbit IgG). Validated for Western blot. Reactivity: Recombinant protein. Recognizes insulin-like growth factor 1. Observed band in Western blot: about 10 kDa.
Target IGF1
Clone number DBG-9
Host Rabbit
Reactivity Recombinant protein
Isotype Rabbit IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M00148 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 uL/vial — Unconjugated
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
      Form: Liquid
      Applications: WB
      Application details: WB 1:500-1:2000
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target IGF1
Alternative names Insulin-like growth factor I;IGF-I;Mechano growth factor;MGF;Somatomedin-C;IGF1;IBP1;
UniProt # P05019
Host Rabbit
Clone DBG-9
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Recombinant protein
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human IGF1
Molecular weight 21.8 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Secreted.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M00148
Main SKU BHA21007817
Rabbit Monoclonal

Product Overview

Clone DBG-9 is a rabbit monoclonal antibody that recognizes insulin-like growth factor 1 (gene IGF1) in Recombinant protein samples and is validated for Western blot. The predicted molecular weight is 21.8 kDa, and the supplier reports an observed band at about 10 kDa.

Insulin-like growth factor 1 (IGF-1) is a peptide hormone, produced mainly by the liver under growth hormone control, that is structurally related to insulin. Acting through the IGF-1 receptor, it promotes cell growth, proliferation and survival and mediates many effects of growth hormone on body growth and metabolism.

Target Insulin-like growth factor 1 (gene IGF1; UniProt P05019, human)
Clone DBG-9
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Recombinant protein
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 21.8 kDa
Observed MW 10 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Immunogen

Synthetic peptide based on the sequence of human IGF1.

Reactivity Notes

The supplier lists reactivity with Recombinant protein. UniProt describes insulin-like growth factor 1 as a secreted protein.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Recombinant protein. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 21.8 kDa; observed band at about 10 kDa. The observed band is much smaller than the calculated size, which can indicate a processed form or a shorter isoform; check the supplier images.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human IGF1.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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