| Field | Specification |
|---|---|
| Target | |
| Alternative names | Insulin-like growth factor 1 receptor;2.7.10.1;Insulin-like growth factor I receptor;IGF-I receptor;CD221;Insulin-like growth factor 1 receptor alpha chain;Insulin-like growth factor 1 receptor beta chain;IGF1R; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human IGF1 Receptor, different from the related rat and mouse sequences by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes insulin-like growth factor 1 receptor (gene IGF1R) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 154.8 kDa; the supplier reports observed bands at 110, 200 kDa.
The IGF-1 receptor (IGF1R) is a receptor tyrosine kinase that binds IGF-1 with high affinity and IGF-2 and insulin more weakly. Ligand binding triggers autophosphorylation and recruitment of adaptors such as IRS proteins and SHC, activating the PI3K-AKT and MAPK pathways that drive growth and survival, which makes IGF1R a focus of growth and cancer research.
| Target | Insulin-like growth factor 1 receptor (gene IGF1R; UniProt P08069, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 154.8 kDa |
| Observed MW | 110, 200 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human SH-SY5Y cells, human A549 cells, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue, rat colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human IGF1 Receptor. It differs from the rat and mouse sequences at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places insulin-like growth factor 1 receptor in the cell membrane. Tissue expression noted by UniProt (human): Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Xing Ren et al. (2024) Network pharmacology, transcriptomics, and biological validation reveal a lipid secretion inhibitory and anti-inflammatory mechanism of tanreqing gel in the treatment of acne. Journal of Ethnopharmacology. 10.1016/j.jep.2024.119278
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Anup S Ramdhave et al. (2017) Energy intake correlates with the levels of fatty acid synthase and insulin-like growth factor-1 in male and female C57BL/6 mice. American Journal of Translational Research. PMID: 28386316
Lu Yan et al. (2017) Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions. International Journal of Molecular Medicine. 10.3892/ijmm.2017.3117
Gong Lei et al. (2021) Circ_0005033 is an oncogene in laryngeal squamous cell carcinoma and regulates cell progression and Cisplatin sensitivity via miR-107/IGF1R axis. Anti-Cancer Drugs. 10.1097/CAD.0000000000001260
Li Dong et al. (2019) Regulation of insulin resistance by targeting the insulin-like growth factor 1 receptor with microRNA-122-5p in hepatic cells. Cell Biology International. 10.1002/cbin.11129
Yang Zhao et al. (2021) Papillary Thyroid Carcinoma in Patients with Acromegaly from a Single Center in China. World Neurosurgery. 10.1016/j.wneu.2021.02.101
L Zhang et al. (2017) miR-182 selectively targets HOXA10 in goat endometrial epithelium cells in vitro. Reproduction in Domestic Animals. 10.1111/rda.13031
Yue Zhang et al. (2017) Effects of interferon tau on endometrial epithelial cells in caprine in vitro. Gene Expression Patterns. 10.1016/j.gep.2017.06.010
