| Field | Specification |
|---|---|
| Target | |
| Alternative names | NF-kappa-B inhibitor alpha;I-kappa-B-alpha;IkB-alpha;IkappaBalpha;Major histocompatibility complex enhancer-binding protein MAD3;NFKBIA;IKBA, MAD3, NFKBI; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IKB alpha |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HO-14 is a rabbit monoclonal antibody that recognizes NF-kappa-B inhibitor alpha (gene NFKBIA) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 35.6 kDa; the supplier reports observed bands at 36, 39 kDa.
IκBα (NFKBIA) holds NF-κB dimers such as p65/p50 in the cytoplasm by hiding the signals they need for nuclear import. When inflammatory stimuli activate the IKK complex, IκBα is phosphorylated and degraded, releasing NF-κB into the nucleus, so its phosphorylation and loss are standard readouts of NF-κB activation.
| Target | NF-kappa-B inhibitor alpha (gene NFKBIA; UniProt P25963, human) |
|---|---|
| Clone | HO-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.6 kDa |
| Observed MW | 36, 39 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
Samples with a confirmed band (WB): human HeLa cells, human Raji cells, human PC-3 cells, human Jurkat cells, rat liver tissue, rat C6 cells, mouse liver tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Samples with confirmed staining (IHC): mouse stomach tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human IKB alpha.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places NF-kappa-B inhibitor alpha in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Man Sun et al. (2025) In Situ Programming of the Tumor Microenvironment to Alleviate Immunosuppression for Pancreatic Cancer Immunotherapy. Advanced Science. 10.1002/advs.202504008
Rui Tang et al. (2026) Exosome membrane-biomimetic nanomedicine targets the pre-metastatic niche via NF-κB inhibition to suppress breast cancer lung metastasis. Materials Today Bio. 10.1016/j.mtbio.2026.102856
Shunying Li et al. (2026) Mechanisms of Mulberry Leaf Extracts Ameliorating Inflammation and Fibrosis in Non-Alcoholic Fatty Liver Disease Mice via NF-κB and TGFβ1/Smad3 Pathways. Phytotherapy Research. 10.1002/ptr.70357
Gang Chen et al. (2024) Sophoridine exerts anti-arthritic effects on fibroblast-like synoviocytes and collagen-induced arthritis in rats. Phytotherapy Research. 10.1002/ptr.8205
Chuang Liu et al. (2022) An Acidic Polysaccharide with Anti-Inflammatory Effects from Blackened Jujube: Conformation and Rheological Properties. Foods. 10.3390/foods11162488
Qi Li et al. (2016) Low doses of paclitaxel enhance liver metastasis of breast cancer cells in the mouse model. FEBS Journal. 10.1111/febs.13767
Xiaoqing Zhu et al. (2024) Anti-inflammatory properties of ophioglonin derived from the fern Ophioglossum vulgatum L. via inactivating NF-κB and MAPK signaling pathways. FEBS Open Bio. 10.1002/2211-5463.13914
Dou Wang et al. (2021) Xingnao Jieyu Decoction Reduces Neuroinflammation through the NF-κB Pathway to Improve Poststroke Depression. Evidence-based Complementary and Alternative Medicine. 10.1155/2021/8629714
Huanyue Cui et al. (2021) Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways. Evidence-based Complementary and Alternative Medicine. 10.1155/2021/6103158
Yang Duan et al. (2019) PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF-B Signaling Pathway. Biomed Research International. 10.1155/2019/8650846
