| Field | Specification |
|---|---|
| Target | |
| Alternative names | NF-kappa-B inhibitor beta; NF-kappa-BIB; I-kappa-B-beta; IkB-B; IkB-beta; IkappaBbeta; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9; NFKBIB; IKBB; TRIP9 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human IkB Beta/NFKBIB recombinant protein (Position: T96-V356). Human NFKBIB shares 79.5% and 76.4% amino acid (aa) sequence identity with mouse and rat NFKBIB, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes NF-kappa-B inhibitor beta (gene NFKBIB) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
IκBβ (NFKBIB) is an inhibitor of NF-κB that traps NF-κB dimers in the cytoplasm. Compared with IκBα it is degraded more slowly after stimulation, and a newly made, unphosphorylated form can accompany NF-κB into the nucleus, which helps shape prolonged NF-κB responses.
| Target | NF-kappa-B inhibitor beta (gene NFKBIB; UniProt Q15653, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human IkB Beta/NFKBIB fragment (Thr96–Val356), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 79.5% and 76.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places NF-kappa-B inhibitor beta in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in all tissues examined.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
