| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NF-kappa-B inhibitor beta;NF-kappa-BIB;I-kappa-B-beta;IkB-B;IkB-beta;IkappaBbeta;Thyroid receptor-interacting protein 9;TR-interacting protein 9;TRIP-9;NFKBIB;IKBB, TRIP9; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human IKB beta |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-NFKBIB antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone FDH-14; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, IP (as provided in the source record). Boster Bio Anti-IKB beta Rabbit Monoclonal Antibody catalog # M05766. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: NFKBIB (NF-kappa-B inhibitor beta).
- Antibody format: Monoclonal; clone FDH-14; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
NFKBIB (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA- dependent inactivation. Association with inhibitor kappa B- interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation. Reported cellular localization context: Cytoplasm . Nucleus . Tissue expression notes (as provided): Expressed in all tissues examined.
Research relevance and current trends
- Research context keywords from the source record include: Epigenetics and Nuclear Signaling,Immunology,Innate Immunity,NFKB Pathway,Nuclear Signaling,Nuclear Signaling Pathways,Signal Transduction,Signaling Pathway,TLR Signaling.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate NFKBIB antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect NFKBIB expression by Western blot in cell or tissue lysates, Detect NFKBIB in FFPE tissue sections by immunohistochemistry, Localize NFKBIB by immunofluorescence/immunocytochemistry in cultured cells, Enrich NFKBIB by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 30 kDa; calculated MW: 37771 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 30 kDa
- Cellular localization (provided): Cytoplasm . Nucleus .
- Tissue details (provided): Expressed in all tissues examined.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
