| Field | Specification |
|---|---|
| Target | |
| Alternative names | Zinc finger protein Aiolos; Ikaros family zinc finger protein 3; IKZF3; ZNFN1A3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IKZF3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22I22 is a rabbit monoclonal antibody that recognizes zinc finger protein Aiolos (gene IKZF3) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 58.0 kDa, and the supplier reports an observed band at about 70 kDa.
Aiolos (IKZF3) is a zinc finger transcription factor of the Ikaros family expressed mainly in lymphocytes. It regulates B-cell differentiation and maturation and influences T-cell survival through BCL2, and it is a known target of degradation by the immunomodulatory drugs lenalidomide and pomalidomide.
| Target | Zinc finger protein Aiolos (gene IKZF3; UniProt Q9UKT9, human) |
|---|---|
| Clone | 22I22 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 58.0 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human Ramos cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human IKZF3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places zinc finger protein Aiolos in the nucleus, cytoplasm and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
