| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; p55; CD antigen CD25; Il2ra; Il2r |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of mouse IL-2 Receptor alpha/Il2ra, which shares 64.3% and 86.7% amino acid (aa) sequence identity with human and rat IL-2 Receptor alpha/Il2ra, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects interleukin-2 receptor subunit alpha (gene Il2ra) in mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 30.7 kDa, and the supplier reports an observed band at about 50 kDa.
IL-2 receptor alpha (CD25) is the subunit that gives the IL-2 receptor high affinity for IL-2. It is highly expressed on regulatory T cells and on activated T cells, and it is essential for Treg maintenance and immune tolerance. CD25 is a common marker for Tregs and T-cell activation, and its soluble form is measured in inflammation studies.
| Target | Interleukin-2 receptor subunit alpha (gene Il2ra; UniProt P01590, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 30.7 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
Samples with a confirmed band (WB): mouse ANA-1 cells, mouse NIH/3T3 cells, mouse RAW264.7 cells, mouse HEPA1–6 cells, rat C6 cells, rat NRK cells, rat RH35 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue, rat small intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HEPA1–6 cells.
Immunogen
Synthetic peptide from the middle region of mouse IL-2 Receptor alpha/Il2ra. Sequence identity with the human and rat orthologs: 64.3% and 86.7%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places interleukin-2 receptor subunit alpha in the membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wong Victoria A. et al. (2024) IL-2Rα KO mice exhibit maternal microchimerism and reveal nuclear localization of IL-2Rα in lymphoid and non-lymphoid cells. Frontiers in Immunology. 10.3389/fimmu.2024.1369818
Hua-qi Hu et al. (2025) Yinqiao antipruritic formula attenuates atopic dermatitis-like skin lesions through modulating the cytokine-cytokine receptor interaction pathway. Fitoterapia. 10.1016/j.fitote.2025.106529
