| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Beta-2-microglobulin; B2m |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouse IL-21/Il21 recombinant protein (Position: H18-S146). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-IL-21/Il21 Antibody is an antibody for Il21 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Il21 (beta-2 microglobulin); UniProt: Q9ES17
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 65-80 kDa, calculated 22875 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-IL-21/Il21 Antibody Picoband® catalog # A00459-1.
Biological background
Biological context: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Expression and localization notes: cellular localization: Secreted . Upon binding to the SLC22A17 (24p3R) receptor, it is internalized., tissue context: Detected in lung, spleen, uterus, vagina and epididymis. ..
Common research applications
- Western blotting (WB): Compare Il21 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Il21 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Il21-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Interleukin 21 (IL-21) is a protein that in humans is encoded by the IL21 gene. This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Secreted . Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
- Tissue details: Detected in lung, spleen, uterus, vagina and epididymis. .
- Research category: Immunoglobulins,Immunology
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
