| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-33; IL-33; Il33 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IL-33 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28I14 is a rabbit monoclonal antibody that recognizes interleukin-33 (gene Il33) in mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 30.0 kDa, and the supplier reports an observed band at about 31 kDa.
Interleukin-33 (IL-33) is an IL-1 family cytokine that acts as an “alarmin” released from damaged epithelial and endothelial cells. Binding to its receptor ST2 (IL1RL1) activates NF-κB and MAPK signaling, promoting type 2 immunity through Th2 cells, mast cells, eosinophils and group 2 innate lymphoid cells. Inside the nucleus, full-length IL-33 also associates with chromatin.
| Target | Interleukin-33 (gene Il33; UniProt Q8BVZ5, mouse) |
|---|---|
| Clone | 28I14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 30.0 kDa |
| Observed MW | 31 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): mouse lung cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human IL-33.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places interleukin-33 in the nucleus, chromosome, cytoplasm and cytoplasmic vesicle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
