Anti-IL15 Antibody Picoband®

SKU:BHA21000136
Research Validated
—
Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-IL15 antibody (Rabbit polyclonal, Rabbit IgG). Validated for ELISA and Western blot. Reactivity: Human. Recognizes interleukin-15. Observed band in Western blot: about 15 kDa. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target IL15
Host Rabbit
Reactivity Human
Isotype Rabbit IgG
Application(s) ELISA, WB
Options selector
Catalog no. Size Conjugation
PB9244 100 ug/vial
Available Options

Select the variant that best fits your experiment.

  • Options:
    • 100 ug/vial / Carrier Free, Unconjugated
      Form: Lyophilized
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
      Applications: ELISA,WB
      Application details: ELISA, 0.1-0.5μg/ml, -
      Western blot, 0.1-0.5μg/ml, Human
      Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
    • 100 ug/vial / APC, Biotin, Cy3, FITC, Fluoro488, Fluoro550, Fluoro594, Fluoro647, PE
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Applications: WB,IHC,ELISA; Flow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cellsContents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 100 ug/vial / HRP
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: varies by selected option; see option details under Options.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target IL15
Alternative names Interleukin-15;IL-15;IL15;
UniProt # P40933
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
Applications
  • ELISA
  • Western Blot
Immunogen E.coli-derived human IL-15 recombinant protein (Position: N49-S162). Human IL-15 shares 70% amino acid (aa) sequence identity with both mouse and rat IL-15.
Molecular weight 18.1 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Isoform IL15-S48AA: Secreted.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) PB9244
Main SKU BHA21000136
Rabbit Polyclonal · Picoband®

Product Overview

This rabbit polyclonal antibody detects interleukin-15 (gene IL15) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 18.1 kDa, and the supplier reports an observed band at about 15 kDa.

Interleukin-15 (IL-15) is a cytokine that signals through the shared IL-2/IL-15Rβ and common γ chains, often presented in trans by IL-15Rα on neighboring cells. It is essential for the development and survival of NK cells and memory CD8+ T cells, so it is widely studied in antiviral and anti-tumor immunity.

Target Interleukin-15 (gene IL15; UniProt P40933, human)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Human
Form Lyophilized
Formulation Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers
Calculated MW 18.1 kDa
Observed MW 15 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

ELISA 0.1–0.5 µg/mL
Western blot 0.1–0.5 µg/mL (Human)

Immunogen

Recombinant human IL-15 fragment (Asn49–Ser162), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 70%.

Reactivity Notes

The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Most abundant in placenta and skeletal muscle.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.1–0.5 µg/mL (Human); ELISA 0.1–0.5 µg/mL. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.Is a blocking reagent available for this antibody?
A.A matching blocking reagent for competition (specificity) controls can be supplied on request. Contact info@biohippo.com to request it.
Q.Is a carrier-free (BSA-free) version available, for example for conjugation?
A.Yes. A carrier-free formulation without BSA, stabilized with trehalose and/or glycerol, can be prepared on request and is compatible with common conjugation chemistries. Please note this when ordering or contact info@biohippo.com. For storage at −20 °C, keep a cryoprotectant such as glycerol in the buffer rather than PBS alone.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 18.1 kDa; observed band at about 15 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Recombinant human IL-15 fragment (Asn49–Ser162), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 70%.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Xu Huan et al. (2021) Development of IL-15/IL-15Rα sushi domain-IgG4 Fc complexes in Pichia pastoris with potent activities and prolonged half-lives. Microbial Cell Factories. 10.1186/s12934-021-01605-3

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