| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pro-interleukin-16;Interleukin-16;IL-16;Lymphocyte chemoattractant factor;LCF;IL16; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human IL-16 recombinant protein (Position: S1212-S1332). Human IL-16 shares 86% amino acid (aa) sequence identity with mouse IL-16. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes pro-interleukin-16 (gene IL16) in human samples and is validated for ELISA, IHC, ICC/IF and Western blot. The predicted molecular weight is 141.8 kDa, and the supplier reports an observed band at about 45–55 kDa.
Interleukin-16 (IL-16) is a cytokine released from a larger precursor after caspase-3 cleavage and acts as a chemoattractant for CD4+ cells, including T cells, monocytes and eosinophils. It uses CD4 as a receptor, and the pro-IL-16 precursor also has intracellular roles, including in T-cell cycle control.
| Target | Pro-interleukin-16 (gene IL16; UniProt Q14005, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 141.8 kDa |
| Observed MW | 45–55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
| Western blot | 0.1–0.5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human U937 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): human cord blood.
Immunogen
Recombinant human IL-16 fragment (Ser1212–Ser1332), expressed in E. coli. Sequence identity with the mouse ortholog: 86%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places pro-interleukin-16 in the [Interleukin-16]: Secreted and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): Expressed in hemopoietic tissues, such as resting T-cells, but undetectable during active T-cell proliferation. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Weidan Fang et al. (2021) microRNA-128-3p inhibits CD4+ regulatory T cells enrichment by targeting interleukin 16 in gastric cancer. Bioengineered. 10.1080/21655979.2021.2017566
