| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-1 receptor antagonist protein; IL-1RN; IL-1ra; IRAP; IL1 inhibitor; Il1rn |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived rat IL1RA recombinant protein (Position: H27-Q178). Rat IL1RA shares 77.6% and 90.7% amino acid (aa) sequence identity with human and mouse IL1RA, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 12C13 is a mouse monoclonal antibody that recognizes interleukin-1 receptor antagonist protein (gene Il1rn) in rat samples and is validated for ELISA. The predicted molecular weight is 20.3 kDa.
The interleukin-1 receptor antagonist (IL-1Ra, IL1RN) is a natural inhibitor of IL-1 signaling. It binds IL1R1 without recruiting the co-receptor IL1RAP, blocking the actions of IL-1α and IL-1β, and deficiency of IL1RN causes severe autoinflammation, highlighting its role in keeping IL-1 activity in check.
| Target | Interleukin-1 receptor antagonist protein (gene Il1rn; UniProt P25086, rat) |
|---|---|
| Clone | 12C13 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 20.3 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| ELISA (capture antibody) | 1–5 µg/mL (Rat) |
|---|
Immunogen
Recombinant rat IL1RA fragment (His27–Gln178), expressed in E. coli. Sequence identity with the human and mouse orthologs: 77.6% and 90.7%, respectively.
Reactivity Notes
The supplier lists reactivity with rat. UniProt describes interleukin-1 receptor antagonist protein as a secreted protein.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
