| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Major vault protein; MVP; Lung resistance-related protein; MVP; LRP |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived rat IL1RA recombinant protein (Position: H27-Q178). Rat IL1RA shares 77.6% and 90.7% amino acid (aa) sequence identity with human and mouse IL1RA, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-IL1RA Antibody (monoclonal, 12C13) is an antibody for Il1rn detection raised in Mouse (Monoclonal, clone Clone: 12C13, Mouse IgG2b), with reported reactivity: Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Il1rn (major vault protein); UniProt: P25086
- Antibody format: Mouse, Monoclonal, clone Clone: 12C13, Mouse IgG2b
- Molecular weight: 100-110 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-IL1RA Antibody Picoband® (monoclonal, 12C13) catalog # M00651-1.
Biological background
Biological context: Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates IFNG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
Expression and localization notes: cellular localization: nuclear pore complex; Cytoplasm; perinuclear region, tissue context: Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells..
Common research applications
- Western blotting (WB): Compare Il1rn levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Il1rn in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Il1rn-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: nuclear pore complex; Cytoplasm; perinuclear region
- Tissue details: Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
- Research category: Cardiogenesis,Cardiovascular,2339,DNA/RNA,Epigenetics and Nuclear Signaling,Transcription Factors/Regulators
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
