| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-1 receptor antagonist protein;IL-1RN;IL-1ra;IRAP;ICIL-1RA;IL1 inhibitor;Anakinra;IL1RN;IL1F3, IL1RA; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IL1RA |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GEI-9 is a rabbit monoclonal antibody that recognizes interleukin-1 receptor antagonist protein (gene IL1RN) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 20.1 kDa, and the supplier reports an observed band at about 20 kDa.
The interleukin-1 receptor antagonist (IL-1Ra, IL1RN) is a natural inhibitor of IL-1 signaling. It binds IL1R1 without recruiting the co-receptor IL1RAP, blocking the actions of IL-1α and IL-1β, and deficiency of IL1RN causes severe autoinflammation, highlighting its role in keeping IL-1 activity in check.
| Target | Interleukin-1 receptor antagonist protein (gene IL1RN; UniProt P18510, human) |
|---|---|
| Clone | GEI-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 20.1 kDa |
| Observed MW | 20 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): HeLa cells, 3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human IL1RA.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): The intracellular form of IL1RN is predominantly expressed in epithelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mengyao Gao et al. (2024) Pharmacodynamics of Sishen decoction in relieving rheumatoid arthritis: Chemical composition, regulatory pathway and online prediction simulation. Heliyon. 10.1016/j.heliyon.2024.e37257
