Anti-IL32 Antibody (monoclonal, 2I2)

SKU:BHA21002387
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-IL32 antibody (Mouse monoclonal, clone 2I2, Mouse IgG2b). Validated for ELISA. Reactivity: Human. Recognizes interleukin-32. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target IL32
Clone number 2I2
Host Mouse
Reactivity Human
Isotype Mouse IgG2b
Application(s) WB, IHC, Flow Cytometry, ELISA
Options selector
Catalog no. Size Conjugation
M03286 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial / Carrier Free, 100 ug/vial / Unconjugated: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.; Form: Lyophilized; Applications: ELISA (Cap); Application details: ELISA (Cap), 1-5μg/ml, Human; Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
    • 100 ug/vial / APC, 100 ug/vial / Biotin, 100 ug/vial / Cy3, 100 ug/vial / FITC, 100 ug/vial / Fluoro488, 100 ug/vial / Fluoro550, 100 ug/vial / Fluoro594, 100 ug/vial / Fluoro647, 100 ug/vial / PE: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.; Form: Liquid; Applications: Flow Cytometry, WB,IHC,ELISA; Application details: Flow Cytometry, 1-3μg/1x106 cells; Western blot, 0.25-0.5μg/ml; Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml; ELISA, 0.1-0.5μg/ml; Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing., At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
    • 100 ug/vial / HRP: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.; Form: Liquid; Applications: WB,IHC,ELISA; Application details: Western blot, 0.25-0.5μg/ml; Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml; ELISA, 0.1-0.5μg/ml; Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
  • Lead time: varies by selected option; please contact us for current fulfillment timing.
  • Storage: varies by selected option; see option details above.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target IL32
Alternative names Interleukin-32; IL-32; Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor; IL32; NK4; TAIF
UniProt # P24001
Host Mouse
Clone 2I2
Clonality
  • Monoclonal
Isotype
  • Mouse IgG2b
Reactivity
  • Human
Applications
  • ELISA
Immunogen E. coli-derived human IL32 recombinant protein (Position: A31-K234).
Molecular weight 26.7 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Cellular localization Secreted
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M03286
Main SKU BHA21002387
Mouse Monoclonal

Product Overview

Clone 2I2 is a mouse monoclonal antibody that recognizes interleukin-32 (gene IL32) in human samples and is validated for ELISA. The predicted molecular weight is 26.7 kDa.

Interleukin-32 (IL-32) is a cytokine found in humans but not in rodents. It induces other inflammatory cytokines such as TNF and IL-8 and activates NF-κB and p38 MAPK signaling, and it is studied in innate and adaptive immune responses and chronic inflammation.

Target Interleukin-32 (gene IL32; UniProt P24001, human)
Clone 2I2
Host / Clonality / Isotype Mouse / Monoclonal / Mouse IgG2b
Reactivity Human
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3
Calculated MW 26.7 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

ELISA (capture antibody) 1–5 µg/mL (Human)

Immunogen

Recombinant human IL32 fragment (Ala31–Lys234), expressed in E. coli.

Reactivity Notes

The supplier lists reactivity with human. UniProt describes interleukin-32 as a secreted protein. Tissue expression noted by UniProt (human): Selectively expressed in lymphocytes.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: ELISA (capture antibody) 1–5 µg/mL (Human). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.Has this antibody been tested on porcine samples?
A.Reactivity with porcine samples has not been tested by the supplier. Aligning the immunogen sequence with the porcine ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.What immunogen was used to raise this antibody?
A.Recombinant human IL32 fragment (Ala31–Lys234), expressed in E. coli.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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