Anti-IL32 Antibody (monoclonal, 2I2)

Overview

Anti-IL32 Antibody (monoclonal, 2I2) is an antibody for IL32 detection raised in Mouse (Monoclonal, clone Clone: 2I2, Mouse IgG2b), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: IL32 (interleukin 32); UniProt: P24001
• Antibody format: Mouse, Monoclonal, clone Clone: 2I2, Mouse IgG2b
• Molecular weight: 130 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-IL32 Antibody Picoband® (monoclonal, 2I2) catalog # M03286.

Biological background

Biological context: Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.

Expression and localization notes: cellular localization: Secreted, tissue context: Expressed in all tissues, but more abundant in heart, brain and skeletal muscle..

Common research applications

• Western blotting (WB): Compare IL32 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of IL32 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify IL32-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Specificity: No cross reactivity with other proteins.
• Background: Interleukin 32 (Il32) is a protein that in humans is encoded by the IL32 gene. This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Secreted
• Tissue details: Expressed in all tissues, but more abundant in heart, brain and skeletal muscle.
• Research category: ER Proteins,Immunology,Protein Trafficking,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.