| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interleukin-4;IL-4;B-cell stimulatory factor 1;BSF-1;Binetrakin;Lymphocyte stimulatory factor 1;Pitrakinra;IL4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human IL4 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-IL4 Rabbit Monoclonal Antibody is an antibody targeting IL4. Common applications include WB, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AADD-9; isotype: Rabbit IgG; reactivity: Human; observed MW: 15 kDa; calculated MW: 17 kDa.
Boster Bio Anti-IL4 Rabbit Monoclonal Antibody catalog # M00230. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Key elements and design rationale
- Target: IL4 — Interleukin-4
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AADD-9; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 15 kDa; Calculated: 17 kDa
Biological background
Protein function (datasheet): Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
Cellular localization (datasheet): Secreted.
Tissue details (datasheet): Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
Research relevance and current trends
- Commonly studied in contexts related to Adaptive Immunity,Cancer,Cytokines,Immunology,Innate Immunity,Interleukins,Tumor Immunology.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
