| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interleukin-7;IL-7;Il7;Il-7; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived mouse IL7 recombinant protein (Position: E26-I154). Mouse IL7 shares 56.4% and 87.6% amino acid (aa) sequence identity with human and rat IL7, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Il7 (Interleukin-7) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-IL7 Antibody Picoband® catalog # PB9871. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived mouse IL7 recombinant protein (Position: E26-I154). Mouse IL7 shares 56.4% and 87.6% amino acid (aa) sequence identity with human and rat IL7, respectively. (reported region: E26-I154).
- Molecular weight context: reported MW: 20 kDa; calculated MW: 17727 MW
- Reactivity: Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Interleukin-7; Interleukin-7. Interleukin 7 (IL-7) is a protein that in humans is encoded by the IL7 gene. The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. And it is found to be a cofactor for V (D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. In addition, this cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed. Functional note: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Reported localization: Secreted. Expression/tissue context: Expressed in skin, thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland and trachea, but not detected in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal gland (PubMed:19384417). In the skin, mainly expressed in stratum granulosum of the epidermis (PubMed:1429717) (PubMed:19384417). .
Research relevance and current trends
- Cytokines: Researchers commonly examine how Il7 (Interleukin-7) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how Il7 (Interleukin-7) relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how Il7 (Interleukin-7) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Il7 (Interleukin-7) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of Il7 (Interleukin-7) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
