| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin enhancer-binding factor 2; Nuclear factor of activated T-cells 45 kDa; ILF2; NF45 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ILF2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18I48 is a rabbit monoclonal antibody that recognizes interleukin enhancer-binding factor 2 (gene ILF2) in human, mouse and rat samples and is validated for Western blot, IHC, immunoprecipitation and flow cytometry. The predicted molecular weight is 43.1 kDa, and the supplier reports an observed band at about 43 kDa.
NF45 (encoded by ILF2) is a nuclear RNA-binding protein that forms a stable heterodimer with NF90/ILF3. The NF45–NF90 complex influences transcription, mRNA stability and cell cycle progression, and it takes part in antiviral responses. NF45 is studied in gene regulation, cell proliferation and host–virus interactions.
| Target | Interleukin enhancer-binding factor 2 (gene ILF2; UniProt Q12905, human) |
|---|---|
| Clone | 18I48 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.1 kDa |
| Observed MW | 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): human A431 cells, human PC-3 cells, human SH-SY5Y cells, human K562 cells, rat brain tissue, mouse brain tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, human lymphoma tissue, human glioma tissue, human testis cancer tissue, human bladder cancer tissue, human renal cancer tissue, human cervical cancer tissue, human colon cancer tissue, human thyroid cancer tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human ILF2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places interleukin enhancer-binding factor 2 in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
