| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin-linked protein kinase;2.7.11.1;59 kDa serine/threonine-protein kinase;ILK-1;ILK-2;p59ILK;ILK;ILK1, ILK2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ILK |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FHE-9 is a rabbit monoclonal antibody that recognizes scaffold protein ILK in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 51.4 kDa, and the supplier reports an observed band at about 51 kDa.
Integrin-linked kinase (ILK) is a scaffold protein bound to the cytoplasmic tails of beta integrins at focal adhesions. With PINCH and parvin it forms the IPP complex that links integrins to the actin cytoskeleton, supporting cell adhesion, spreading and migration. ILK is studied in cell–matrix signaling, tissue development and cancer cell motility.
| Target | Scaffold protein ILK (gene ILK; UniProt Q13418, human) |
|---|---|
| Clone | FHE-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 51.4 kDa |
| Observed MW | 51 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human Caco-2 cells, human U2OS cells, human A549 cells, human HepG2 cells, human 293T cells, rat brain tissue, rat kidney tissue, rat spleen tissue, rat PC-12 cells, mouse spleen tissue, mouse heart tissue, Rabbit-IgG, mouse brain tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Samples with confirmed staining (IHC): human stomach tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): 293 cells.
Immunogen
Synthetic peptide based on the sequence of human ILK.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places scaffold protein ILK in the cell junction, cell membrane and cell projection. Tissue expression noted by UniProt (human): Highly expressed in heart followed by skeletal muscle, pancreas and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Bin Qiao et al. (2017) MicroRNA-542-3p inhibits oral squamous cell carcinoma progression by inhibiting ILK/TGF-β1/Smad2/3 signaling. Oncotarget. 10.18632/oncotarget.19986
