| Field | Specification |
|---|---|
| Target | |
| Alternative names | U3 small nucleolar ribonucleoprotein protein IMP4; U3 snoRNP protein IMP4; Brix domain-containing protein 4; IMP4; BXDC4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derivedhumanIMP4recombinantprotein(Position:L2-K236).HumanIMP4?shares?97.9%?amino?acid?(aa)?sequence?identity?withbothmouse?and?ratIMP4. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes U3 small nucleolar ribonucleoprotein protein IMP4 in human, mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 33.8 kDa, and the supplier reports an observed band at about 36 kDa.
IMP4 is a nucleolar protein of the U3 small nucleolar ribonucleoprotein (snoRNP) and part of the small subunit processome. It is needed for the early cleavage steps of pre-ribosomal RNA that produce 18S rRNA, and so it contributes to assembly of the small ribosomal subunit.
| Target | U3 small nucleolar ribonucleoprotein protein IMP4 (gene IMP4; UniProt Q96G21, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 33.8 kDa |
| Observed MW | 36 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human MCF-7 cells, rat PC-12 cells, mouse thymus tissue, mouse RAW264.7 cells, mouse EL-4 cells, mouse Ana-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
E. coli-derivedhumanIMP4recombinantprotein(Position:L2-K236).HumanIMP4?shares?97.9%?amino?acid?(aa)?sequence?identity?withbothmouse?and?ratIMP4.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places U3 small nucleolar ribonucleoprotein protein IMP4 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
