| Field | Specification |
|---|---|
| Target | |
| Alternative names | Inosine-5'-monophosphate dehydrogenase 2; IMP dehydrogenase 2; IMPD 2; IMPDH 2; Inosine-5'-monophosphate dehydrogenase type II; IMP dehydrogenase II; IMPDH-II; IMPDH2; IMPD2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IMPDH2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20I47 is a rabbit monoclonal antibody that recognizes inosine-5'-monophosphate dehydrogenase 2 (gene IMPDH2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 55.8 kDa, and the supplier reports an observed band at about 56 kDa.
IMPDH2 (inosine-5′-monophosphate dehydrogenase 2) catalyzes the conversion of IMP to XMP, the rate-limiting step in de novo guanine nucleotide synthesis. It is the predominant isoform in proliferating cells, including activated lymphocytes, and it is the target of immunosuppressive drugs such as mycophenolic acid. It is studied in cell growth, metabolism and cancer.
| Target | Inosine-5'-monophosphate dehydrogenase 2 (gene IMPDH2; UniProt P12268, human) |
|---|---|
| Clone | 20I47 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 55.8 kDa |
| Observed MW | 56 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human IMPDH2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places inosine-5'-monophosphate dehydrogenase 2 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): IMPDH1 is the main species in normal leukocytes and IMPDH2 predominates over IMPDH1 in the tumor. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
