| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interphotoreceptor matrix proteoglycan 2; Interphotoreceptor matrix proteoglycan of 200 kDa; IPM 200; Sialoprotein associated with cones and rods proteoglycan; Spacrcan; IMPG2; IPM200 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derivedhumanIMPG2recombinantprotein(Position:R81-R1080).HumanIMPG2shares70.8%and72%aminoacid(aa)sequenceidentitywithmouseandratIMPG2,respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes interphotoreceptor matrix proteoglycan 2 (gene IMPG2) in human, mouse and rat samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 138.6 kDa, and the supplier reports an observed band at about 150 kDa.
IMPG2 (interphotoreceptor matrix proteoglycan 2) is a proteoglycan secreted into the matrix that surrounds photoreceptor outer segments. It binds chondroitin sulfate and hyaluronan and helps organize this matrix and maintain photoreceptors. Mutations in IMPG2 are associated with inherited retinal dystrophies.
| Target | Interphotoreceptor matrix proteoglycan 2 (gene IMPG2; UniProt Q9BZV3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 138.6 kDa |
| Observed MW | 150 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEL cells, human K562 cells, rat testis tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat retina tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
E. coli-derivedhumanIMPG2recombinantprotein(Position:R81-R1080).HumanIMPG2shares70.8%and72%aminoacid(aa)sequenceidentitywithmouseandratIMPG2,respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places interphotoreceptor matrix proteoglycan 2 in the photoreceptor outer segment membrane and photoreceptor inner segment membrane. Tissue expression noted by UniProt (human): Expressed in the retina (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
