| Field | Specification |
|---|---|
| Target | |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C19H30O3 |
| SMILES | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
Anti-inflammatory agent 31 is an andrographolide derivative with anti-inflammatory activity. It inhibits NF-κB activation through upstream blockade of PI3K/Akt and ERK1/2 MAPK signaling, and it restores intracellular GSH levels while exerting a protective effect on the liver[1]. Its molecular formula is C19H30O3, with a molecular weight of 306.44 g/mol.
Physical & Chemical Properties
| CAS Number | 3051838-54-8 |
|---|---|
| Molecular Formula | C19H30O3 |
| Molecular Weight | 306.44 g/mol |
| SMILES | CC(/C=C/C[C@@H]1C(CC[C@]2([H])[C@](C)(CO)[C@H](O)CC[C@@]12C)=C)=O |
| Target | ERK1, ERK2 |
| Signaling Pathway | MAPK/ERK Pathway; Stem Cell/Wnt; NF-κB |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with your institution's chemical hygiene plan.
In Vitro
At 100, 200, and 400 μM for 24 h, Anti-inflammatory agent 31 (enone 17) prevents activated NF-κB p50 subunit from binding DNA in a dose-dependent manner[1]. It reacts at a quick rate (KGSH=0.076 M-1.h-1) and has a long half-life (T1/2=6.47 h)[1]. At 5, 20, and 50 μM for 24 h, it effectively restores intracellular GSH levels, and 5 μM raises GSH levels more than 2.5-fold over basal levels[1]. In LO2 and HEK293 cells, it shows low cytotoxicity at 5, 20, and 50 μM (24 h), with CC50 of 14.68 and 26.25 μM, respectively[1].
Immunofluorescence[1]
| Cell Line | RAW-blueTM cells |
|---|---|
| Concentration | 5, 10, 20 μM |
| Incubation Time | 24 hours |
| Result | Inhibited the LPS-induced NF-κB activity and indicated the anti-inflammatory properties. |
In Vivo
In mice, Anti-inflammatory agent 31 (enone 17) (i.p.; 0.3, 1.0 mg/kg) lowers total white blood cell and neutrophil counts and reduces LPS-induced acute lung injury in a dose-dependent manner[1].
| Animal Model | LPS-induced acute lung injury (ALI) mouse model (female BALB/c mice; 7-week-old)[1] |
|---|---|
| Dosage | 0.3, 1.0 mg/kg |
| Administration | Intraperitoneal injection 1 h after LPS challenge; twice daily for 4 days |
| Result | Alleviated LPS-induced acute lung injury. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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