| Field | Specification |
|---|---|
| Target | |
| Alternative names | Inhibitor of growth protein 1; ING1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ING1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28I93 is a rabbit monoclonal antibody that recognizes inhibitor of growth protein 1 (gene ING1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 46.7 kDa, and the supplier reports an observed band at about 47 kDa.
ING1 (inhibitor of growth 1) is the founding member of the ING family of chromatin-associated proteins that contain a PHD finger recognizing histone H3K4me3. It works with p53 to restrain cell growth and promote apoptosis, and it is regarded as a candidate tumor suppressor in cancer research.
| Target | Inhibitor of growth protein 1 (gene ING1; UniProt Q9UK53, human) |
|---|---|
| Clone | 28I93 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.7 kDa |
| Observed MW | 47 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ING1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places inhibitor of growth protein 1 in the nucleus. Tissue expression noted by UniProt (human): Isoform 2 was expressed in all normal tissues and cells examined, as well as in all breast cancer and melanoma cell lines examined. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
