| Field | Specification |
|---|---|
| Target | |
| Alternative names | Insulin;Insulin B chain;Insulin A chain;INS; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Insulin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DEA-9 is a rabbit monoclonal antibody that recognizes insulin in human, mouse and rat samples and is validated for IHC and ICC/IF. The predicted molecular weight is 12.0 kDa, and the supplier reports an observed band at about 12 kDa.
Insulin is a peptide hormone secreted by pancreatic beta cells that lowers blood glucose. It is made as proinsulin and processed into A and B chains joined by disulfide bonds, releasing C-peptide. By activating the insulin receptor, it promotes glucose uptake in muscle and fat and glycogen and lipid synthesis in the liver, making it central to diabetes and metabolism research.
| Target | Insulin (gene INS; UniProt P01308, human) |
|---|---|
| Clone | DEA-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 12.0 kDa |
| Observed MW | 12 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Immunohistochemistry | 1:50–1:200 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): mouse pancreas tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): Beta-TC-6 cells.
Immunogen
Synthetic peptide based on the sequence of human insulin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes insulin as a secreted protein. Tissue expression noted by UniProt (human): Expressed by pancreatic beta-cells (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Feng-Ya Jing et al. (2021) Enhancing antioxidant and anti-hyperglycaemic functions of gingko biloba L. seeds using thermal detoxification. Journal of Functional Foods. 10.1016/j.jff.2021.104819
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Yin Xiao-Lu et al. (2018) Gynura divaricata rich in 3, 5−/4, 5-dicaffeoylquinic acid and chlorogenic acid reduces islet cell apoptosis and improves pancreatic function in type 2 diabetic mice. Nutrition & Metabolism. 10.1186/s12986-018-0310-y
Wang Lu et al. (2019) Phenotypic characterization of a novel type 2 diabetes animal model in a SHANXI MU colony of Chinese hamsters. Endocrine. 10.1007/s12020-019-01940-x
Tianle Gao et al. (2019) Protective Effects of Konjac and Inulin Extracts on Type 1 and Type 2 Diabetes. Journal of Diabetes Research. 10.1155/2019/3872182
