| Field | Specification |
|---|---|
| Target | |
| Alternative names | Insulin receptor substrate 1;IRS-1;Irs1;Irs-1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse IRS1, identical to the related rat sequence, and different from the related human sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets insulin receptor substrate 1 (gene Irs1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 130.7 kDa, and the supplier reports an observed band at about 132 kDa.
Insulin receptor substrate 1 (IRS1) is an adaptor protein phosphorylated on tyrosine by the insulin and IGF-1 receptors. Phosphorylated IRS1 recruits PI3K and GRB2, relaying signals to the AKT and MAPK pathways that control glucose uptake, metabolism and growth. Inhibitory serine phosphorylation of IRS1 is a common readout in insulin resistance research.
| Target | Insulin receptor substrate 1 (gene Irs1; UniProt P35569, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 130.7 kDa |
| Observed MW | 132 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): HeLa cells, A549 cells, 293T cells, MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the N-terminal region of mouse IRS1. The peptide sequence is conserved in rat. It differs from the human sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places insulin receptor substrate 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (mouse): Expressed in osteoblasts, but not in osteoclasts. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Thaís C. Costa et al. (2019) Effect of maternal feed restriction in dairy goats at different stages of gestation on skeletal muscle development and energy metabolism of kids at the time of births. Animal Reproduction Science. 10.1016/j.anireprosci.2019.05.006
