| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-2; CD49 antigen-like family member B; Collagen receptor; Platelet membrane glycoprotein Ia; GPIa; VLA-2 subunit alpha; CD antigen CD49b; ITGA2; CD49B |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Integrin alpha 2/ITGA2 recombinant protein (Position: E285-S1181). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes integrin alpha-2 (gene ITGA2) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 129.3 kDa, and the supplier reports an observed band at about 165 kDa.
Integrin alpha-2 (ITGA2, CD49b) pairs with integrin beta-1 to form a major collagen receptor on platelets, epithelial cells and fibroblasts. It supports platelet adhesion to exposed collagen, cell migration and collagen matrix remodeling, and CD49b is also used as a marker of certain NK and T cell subsets.
| Target | Integrin alpha-2 (gene ITGA2; UniProt P17301, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 129.3 kDa |
| Observed MW | 165 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): A375 cells.
Immunogen
Recombinant human Integrin alpha 2/ITGA2 fragment (Glu285–Ser1181), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places integrin alpha-2 in the membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
de Queiroz Rafaela Muniz et al. (2024) Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53. Nature Communications. 10.1038/s41467-024-51488-2
