| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-3;CD49 antigen-like family member C;FRP-2;Galactoprotein B3;GAPB3;VLA-3 subunit alpha;CD49c;Integrin alpha-3 heavy chain;Integrin alpha-3 light chain;ITGA3;MSK18; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Integrin alpha 3, identical to the related rat and mouse sequences. |
| Molecular weight | |
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| Reconstitution | |
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| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects integrin alpha-3 (gene ITGA3) in human and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 116.6 kDa, and the supplier reports an observed band at about 155 kDa.
Integrin alpha-3 (ITGA3, CD49c) pairs with integrin beta-1 to form a receptor for laminins, especially laminin-332 and laminin-511. It is highly expressed in epithelial cells and supports their adhesion to basement membranes, and it is important for kidney, lung and skin integrity.
| Target | Integrin alpha-3 (gene ITGA3; UniProt P26006, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 116.6 kDa |
| Observed MW | 155 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human U87 cells, human A431 cells, human A549 cells, human U2OS cells, rat C6 cells, rat PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, human colorectal adenocarcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Synthetic peptide from the N-terminal region of human integrin alpha 3. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places integrin alpha-3 in the cell membrane and cell projection. Tissue expression noted by UniProt (human): Isoform 1 is widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xuyang Zhang et al. (2024) Iron/ROS/Itga3 mediated accelerated depletion of hippocampal neural stem cell pool contributes to cognitive impairment after hemorrhagic stroke. Redox Biology. 10.1016/j.redox.2024.103086
Zhou Li et al. (2021) Effect of Irisin on LIF and integrin αvβ3 in rats of implantation failure. Reproductive Biology and Endocrinology. 10.1186/s12958-021-00700-9
Huiling Li et al. (2020) Synthesis and Preclinical Evaluation of a 68Ga-Radiolabeled Peptide Targeting Very Late Antigen-3 for PET Imaging of Pancreatic Cancer. Molecular Pharmaceutics. 10.1021/acs.molpharmaceut.0c00416
