| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-4; CD49 antigen-like family member D; Integrin alpha-IV; VLA-4 subunit alpha; CD antigen CD49d; ITGA4; CD49D |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Integrin Alpha 4/ITGA4 recombinant protein (Position: N35-V269). Human ITGA4 shares 85.5% amino acid (aa) sequence identity with mouse ITGA4. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes integrin alpha-4 (gene ITGA4) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 114.9 kDa, and the supplier reports an observed band at about 140–150kDa.
Integrin alpha-4 (ITGA4, CD49d) pairs with beta-1 to form VLA-4 and with beta-7 to form α4β7. These receptors bind VCAM-1, fibronectin and MAdCAM-1, guiding leukocyte adhesion to blood vessel walls and homing to inflamed tissue and the gut. Alpha-4 integrins are established targets in studies of leukocyte trafficking and inflammation.
| Target | Integrin alpha-4 (gene ITGA4; UniProt P13612, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 114.9 kDa |
| Observed MW | 140–150kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, human THP-1 cells, human HL-60 cells, human U87 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human Integrin Alpha 4/ITGA4 fragment (Asn35–Val269), expressed in E. coli. Sequence identity with the mouse ortholog: 85.5%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places integrin alpha-4 in the membrane. Tissue expression noted by UniProt (human): Expressed in vascular smooth muscle cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
