| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-4;CD49 antigen-like family member D;Integrin alpha-IV;VLA-4 subunit alpha;CD49d;ITGA4;CD49D; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Integrin alpha 4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IHH-9 is a rabbit monoclonal antibody that recognizes integrin alpha-4 (gene ITGA4) in human samples and is validated for Western blot. The predicted molecular weight is 114.9 kDa, and the supplier reports an observed band at about 150 kDa.
Integrin alpha-4 (ITGA4, CD49d) pairs with beta-1 to form VLA-4 and with beta-7 to form α4β7. These receptors bind VCAM-1, fibronectin and MAdCAM-1, guiding leukocyte adhesion to blood vessel walls and homing to inflamed tissue and the gut. Alpha-4 integrins are established targets in studies of leukocyte trafficking and inflammation.
| Target | Integrin alpha-4 (gene ITGA4; UniProt P13612, human) |
|---|---|
| Clone | IHH-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 114.9 kDa |
| Observed MW | 150 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human integrin alpha 4.
Reactivity Notes
The supplier lists reactivity with human. UniProt places integrin alpha-4 in the membrane. Tissue expression noted by UniProt (human): Expressed in vascular smooth muscle cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
