| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-6;CD49 antigen-like family member F;VLA-6;CD49f;Integrin alpha-6 heavy chain;Integrin alpha-6 light chain;Processed integrin alpha-6;Alpha6p;ITGA6; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Integrin alpha 6 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DHG-9 is a rabbit monoclonal antibody that recognizes integrin alpha-6 (gene ITGA6) in human, mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 126.6 kDa, and the supplier reports an observed band at about 127 kDa.
Integrin alpha-6 (ITGA6, CD49f) pairs with beta-1 or beta-4 to form receptors for laminins. The α6β4 form is a core component of hemidesmosomes that anchor epithelial cells to the basement membrane, and CD49f is widely used as a marker of stem and progenitor cells in epithelial and hematopoietic research.
| Target | Integrin alpha-6 (gene ITGA6; UniProt P23229, human) |
|---|---|
| Clone | DHG-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 126.6 kDa |
| Observed MW | 127 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human prostate cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human integrin alpha 6.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places integrin alpha-6 in the cell membrane. Tissue expression noted by UniProt (human): Integrin alpha-6/beta-4 is predominantly expressed by epithelia. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Afonso Malheiro et al. (2020) A three-dimensional biomimetic peripheral nerve model for drug testing and disease modelling. Biomaterials. 10.1016/j.biomaterials.2020.120230
Zhe Xu et al. (2023) Bazi Bushen alleviates skin senescence by orchestrating skin homeostasis in SAMP6 mice. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.17833
