| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-9; Integrin alpha-RLC; ITGA9 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Integrin alpha-9/ITGA9 recombinant protein (Position: Q526-Q1035). Human ITGA9 shares 89.6% amino acid (aa) sequence identity with mouse ITGA9. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects integrin alpha-9 (gene ITGA9) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 114.5 kDa, and the supplier reports an observed band at about 120 kDa.
Integrin alpha-9 (ITGA9) pairs with integrin beta-1 to bind ligands such as tenascin-C, VCAM-1 and osteopontin. It is needed for normal lymphatic valve development, and it contributes to cell adhesion, migration and neutrophil function.
| Target | Integrin alpha-9 (gene ITGA9; UniProt Q13797, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 114.5 kDa |
| Observed MW | 120 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells, human SH-SY5Y cells, human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): SH-SY5Y cells.
Immunogen
Recombinant human Integrin alpha-9/ITGA9 fragment (Gln526–Gln1035), expressed in E. coli. Sequence identity with the mouse ortholog: 89.6%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places integrin alpha-9 in the membrane. Tissue expression noted by UniProt (human): Expressed in vascular smooth muscle cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
