| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-E;HML-1 antigen;Integrin alpha-IEL;Mucosal lymphocyte 1 antigen;CD103;Integrin alpha-E light chain;Integrin alpha-E heavy chain;ITGAE; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Integrin alpha E |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AbI91 is a rabbit monoclonal antibody that recognizes integrin alpha-E (gene ITGAE) in human samples and is validated for IHC. The predicted molecular weight is 130.2 kDa, and the supplier reports an observed band at about 130 kDa.
Integrin alpha-E (ITGAE, CD103) pairs with integrin beta-7 and binds E-cadherin on epithelial cells. It retains intraepithelial lymphocytes and tissue-resident memory T cells within epithelia, and CD103 is also a marker of a migratory dendritic cell subset in the gut and other tissues.
| Target | Integrin alpha-E (gene ITGAE; UniProt P38570, human) |
|---|---|
| Clone | AbI91 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 130.2 kDa |
| Observed MW | 130 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Immunohistochemistry | 1:50–1:200 |
|---|
Samples with confirmed staining (IHC): human cervical cancer, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human integrin alpha E.
Reactivity Notes
The supplier lists reactivity with human. UniProt places integrin alpha-E in the membrane. Tissue expression noted by UniProt (human): Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Ziyu et al. (2023) Immune characteristics associated with lymph node metastasis in early-stage NSCLC. Cellular Oncology. 10.1007/s13402-023-00873-y
Fang Jiang et al. (2022) Single-Cell Profiling of the Immune Atlas of Tumor-Infiltrating Lymphocytes in Endometrial Carcinoma. Cancers. 10.3390/cancers14174311
Huang Jinyu et al. (2013) S100+ cells: A new neuro-immune cross-talkers in lymph organs. Scientific Reports. 10.1038/srep01114
