| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-V;Vitronectin receptor subunit alpha;CD51;Integrin alpha-V heavy chain;Integrin alpha-V light chain;ITGAV;MSK8, VNRA; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Integrin alpha V |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABGC-9 is a rabbit monoclonal antibody that recognizes integrin alpha-V (gene ITGAV) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 116.0 kDa, and the supplier reports an observed band at about 69 kDa.
Integrin alpha-V (ITGAV, CD51) pairs with several beta subunits (β1, β3, β5, β6, β8) to form receptors that recognize the RGD motif in vitronectin, fibronectin, osteopontin and other matrix proteins. Alpha-V integrins also activate latent TGF-β, and they are studied in angiogenesis, fibrosis and tumor biology.
| Target | Integrin alpha-V (gene ITGAV; UniProt P06756, human) |
|---|---|
| Clone | ABGC-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 116.0 kDa |
| Observed MW | 69 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human A549 cells, human A431 cells, human 293T cells, human MCF-7 cells, rat spleen tissue, rat brain tissue, mouse spleen tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human integrin alpha V.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places integrin alpha-V in the cell membrane and cell junction. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tong Wu et al. (2026) Ovarian Matrisome Dynamics and αvβ3-Mediated Regulation in Early Follicular Development. Advanced Science. 10.1002/advs.202507314
Qian Chen et al. (2015) A Fibrin Matrix Promotes the Differentiation of EMSCs Isolated from Nasal Respiratory Mucosa to Myelinating Phenotypical Schwann-Like Cells. Molecules and Cells. 10.14348/molcells.2015.2170
Li Zhou et al. (2026) Irisin Improves Endometrial Receptivity in Obese Rats Through the Wnt/β-Catenin Signaling Pathway. Journal of Obstetrics and Gynaecology Research. 10.1111/jog.70348
