| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin beta-1;Fibronectin receptor subunit beta;Glycoprotein IIa;GPIIA;VLA-4 subunit beta;CD29;ITGB1;FNRB, MDF2, MSK12; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ITGB1 recombinant protein (Position: N527-D728). Human ITGB1 shares 91% and 88% amino acid (aa) sequences identity with mouse and rat ITGB1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects integrin beta-1 (gene ITGB1) in human and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 88.4 kDa, and the supplier reports an observed band at about 120–130 kDa.
Integrin beta-1 (ITGB1, CD29) is the most widely shared integrin beta subunit, pairing with many alpha subunits to form receptors for collagens, laminins, fibronectin and VCAM-1. Beta-1 integrins link the extracellular matrix to the actin cytoskeleton at focal adhesions and drive signaling for cell adhesion, migration, survival and tissue organization.
| Target | Integrin beta-1 (gene ITGB1; UniProt P05556, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 88.4 kDa |
| Observed MW | 120–130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
Samples with a confirmed band (WB): human A431 cells, rat C6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human placenta tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human ITGB1 fragment (Asn527–Asp728), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 91% and 88%, respectively.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places integrin beta-1 in the cell membrane, cell projection, recycling endosome and melanosome. Tissue expression noted by UniProt (human): Expressed in vascular smooth muscle cells (at protein level)... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaoran Zhao et al. (2024) Fibronectin binds integrin α5β1 to regulate macular neovascularization through the Wnt/β-catenin signaling pathway. Experimental Eye Research. 10.1016/j.exer.2024.109880
Xudong Cheng et al. (2014) Astragaloside IV inhibits migration and invasion in human lung cancer A549 cells via regulating PKC-α-ERK1/2-NF-κB pathway. International Immunopharmacology. 10.1016/j.intimp.2014.08.027
Gang Zhao et al. (2010) Mechanical stiffness of liver tissues in relation to integrin β1 expression may influence the development of hepatic cirrhosis and hepatocellular carcinoma. Journal of Surgical Oncology. 10.1002/jso.21613
Shaowei Yang et al. (2016) Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype. International Journal of Molecular Medicine. 10.3892/ijmm.2016.2519
Xu‑Dong Cheng et al. (2015) Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC‑α/ERK1/2 pathway: An in vitro investigation Corrigendum in /10.3892/mmr.2016.4976. Molecular Medicine Reports. 10.3892/mmr.2015.4449
Yuanyi Xu et al. (2016) Inhibition of the peritoneal metastasis of human gastric cancer cells by dextran sulphate in vivo and in vitro. Oncology Letters. 10.3892/ol.2016.4234
