| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin beta-3;Platelet membrane glycoprotein IIIa;GPIIIa;CD61;ITGB3;GP3A; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin beta 3, identical to the related rat and mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects integrin beta-3 (gene ITGB3) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 87.1 kDa, and the supplier reports an observed band at about 29 kDa.
Integrin beta-3 (ITGB3, CD61) pairs with alpha-IIb to form the platelet fibrinogen receptor and with alpha-V to form the vitronectin receptor αVβ3. These integrins mediate platelet aggregation, cell adhesion to RGD-containing matrix proteins, angiogenesis and bone resorption by osteoclasts. Mutations in ITGB3 cause Glanzmann thrombasthenia.
| Target | Integrin beta-3 (gene ITGB3; UniProt P05106, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 87.1 kDa |
| Observed MW | 29 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat) |
Samples with a confirmed band (WB): human U87 cells, human HEL cells, human HeLa cells, rat spleen tissue, mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human laryngeal squamous cells carcinoma tissue, human liver cancer tissue, human thyroid cancer tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human integrin beta 3. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places integrin beta-3 in the cell membrane, cell projection, cell junction and postsynaptic cell membrane. Tissue expression noted by UniProt (human): Isoform beta-3A and isoform beta-3C are widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qin Xing et al. (2016) TGFβ3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis. Scientific Reports. 10.1038/srep20587
Xingsheng Hu et al. (2024) The expression and role of SUZ12 in lung adenocarcinoma. Cancer Medicine. 10.1002/cam4.70190
Yifei Wang et al. (2011) Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin αv, β3 in Epithelial Ovarian Tumors. International Journal of Molecular Sciences. 10.3390/ijms12063409
Changzhi Wang et al. (2011) Overexpression of Lewis (y) Antigen Protects Ovarian Cancer RMG-1 Cells from Carboplatin-Induced Apoptosis by the Upregulation of Topo-I and Topo-II β. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology. 10.1002/ar.21398
