Anti-Integrin beta 5/ITGB5 Antibody Picoband® (monoclonal, 9E2)

Overview

Anti-Integrin beta 5/ITGB5 Antibody Picoband® (monoclonal, 9E2) is an antibody for ITGB5 detection raised in Mouse (Monoclonal, clone Clone: 9E2, Mouse IgG1), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: ITGB5 (heat shock 105kDa/110kDa protein 1); UniProt: P18084
• Antibody format: Mouse, Monoclonal, clone Clone: 9E2, Mouse IgG1
• Molecular weight: 100 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-Integrin beta 5/ITGB5 Antibody Picoband® (monoclonal, 9E2) catalog # M04201.

Biological background

Biological context: Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release. Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.

Expression and localization notes: cellular localization: Cytoplasm., tissue context: Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells..

Common research applications

• Western blotting (WB): Compare ITGB5 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of ITGB5 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify ITGB5-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: This gene encodes a beta subunit of integrin, which can combine with different alpha chains to form a variety of integrin heterodimers. Integrins are integral cell-surface receptors that participate in cell adhesion as well as cell-surface mediated signaling. The alphav beta5 integrin is involved in adhesion to vitronectin.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cytoplasm.
• Tissue details: Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
• Research category: Cell Adhesion Proteins,Epigenetics and Nuclear Signaling,Integrins,Mediator Complex,Neuroscience,Receptors,Transcription

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.