| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin-linked protein kinase;2.7.11.1;59 kDa serine/threonine-protein kinase;ILK-1;ILK-2;p59ILK;ILK;ILK1, ILK2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Integrin linked ILK recombinant protein (Position: M1-H203). Human Integrin linked ILK shares 99.5% amino acid (aa) sequence identity with both mouse and rat Integrin linked ILK. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets scaffold protein ILK in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 51.4 kDa, and the supplier reports an observed band at about 55 kDa.
Integrin-linked kinase (ILK) is a scaffold protein bound to the cytoplasmic tails of beta integrins at focal adhesions. With PINCH and parvin it forms the IPP complex that links integrins to the actin cytoskeleton, supporting cell adhesion, spreading and migration. ILK is studied in cell–matrix signaling, tissue development and cancer cell motility.
| Target | Scaffold protein ILK (gene ILK; UniProt Q13418, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 51.4 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat lung tissue, mouse liver tissue, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human Integrin linked ILK fragment (Met1–His203), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 99.5%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places scaffold protein ILK in the cell junction, cell membrane and cell projection. Tissue expression noted by UniProt (human): Highly expressed in heart followed by skeletal muscle, pancreas and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Guangna Yue et al. (2018) Role of ILK/p38 pathway in mediating the enhanced osteogenic differentiation of bone marrow mesenchymal stem cells on amorphous carbon coating. Biomaterials Science. 10.1039/C8BM01151F
