| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon beta;IFN-beta;Fibroblast interferon;IFNB1;IFB, IFNB; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Interferon beta |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOHB-9 is a rabbit monoclonal antibody that recognizes interferon beta (gene IFNB1) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 22.3 kDa, and the supplier reports an observed band at about 22 kDa.
Interferon beta (IFNB1) is a type I interferon made by many cell types, especially fibroblasts and epithelial cells, when they detect viral infection. It binds the IFNAR1/IFNAR2 receptor and activates JAK-STAT signaling to switch on antiviral and immunoregulatory genes, and it is a central cytokine in innate immunity research.
| Target | Interferon beta (gene IFNB1; UniProt P01574, human) |
|---|---|
| Clone | AOHB-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 22.3 kDa |
| Observed MW | 22 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human interferon beta.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes interferon beta as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
