Anti-Interferon gamma IFNG Antibody (monoclonal, 8E9)

SKU:BHA21001759
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Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-IFNG antibody (Mouse monoclonal, clone Clone: 8E9, Mouse IgG1). Validated for ELISA. Reactivity: Rat. Recognizes interferon gamma. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target IFNG
Clone number Clone: 8E9
Host Mouse
Reactivity Rat
Isotype Mouse IgG1
Application(s) ELISA
Options selector
Catalog no. Size Conjugation
M00393-2 100 ug/vial
Available Options

Select the variant that best fits your experiment.

  • Options:
    • 100 ug/vial / Carrier Free, Unconjugated
      Form: Lyophilized
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
      Applications: ELISA (Cap)
      Application details: ELISA (Cap), 1-5μg/mlContents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
    • 100 ug/vial / APC, Biotin, Cy3, FITC, Fluoro488, Fluoro550, Fluoro594, Fluoro647, PE
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Applications: WB,IHC,ELISA; Flow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cellsContents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 100 ug/vial / HRP
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: varies by selected option; see option details under Options.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target IFNG
Alternative names Interferon gamma; IFN-gamma; IFNG
UniProt # P01581
Host Mouse
Clone Clone: 8E9
Clonality
  • Monoclonal
Isotype
  • Mouse IgG1
Reactivity
  • Rat
Applications
  • ELISA
Immunogen E. coli-derived rat Interferon gamma recombinant protein (Position: Q23-C156). Rat Interferon gamma shares 38% and 86% amino acid (aa) sequence identity with human and mouse Interferon gamma, respectively.
Molecular weight 17.9 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Secreted.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M00393-2
Main SKU BHA21001759
Mouse Monoclonal

Product Overview

Clone Clone: 8E9 is a mouse monoclonal antibody that recognizes interferon gamma (gene Ifng) in rat samples and is validated for ELISA. The predicted molecular weight is 17.9 kDa.

Interferon gamma is the only type II interferon. It is produced mainly by activated T cells and NK cells, signals through IFNGR1/IFNGR2 and the JAK1/JAK2–STAT1 pathway, and activates macrophages and antigen presentation, making it a key readout of Th1 and cytotoxic immune responses.

Target Interferon gamma (gene Ifng; UniProt P01581, rat)
Clone Clone: 8E9
Host / Clonality / Isotype Mouse / Monoclonal / Mouse IgG1
Reactivity Rat
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3
Calculated MW 17.9 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

ELISA (capture antibody) 1–5 µg/mL

Immunogen

Recombinant rat Interferon gamma fragment (Gln23–Cys156), expressed in E. coli. Sequence identity with the human and mouse orthologs: 38% and 86%, respectively.

Reactivity Notes

The supplier lists reactivity with rat. UniProt describes interferon gamma as a secreted protein. Tissue expression noted by UniProt (rat): Released primarily from activated T lymphocytes.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: ELISA (capture antibody) 1–5 µg/mL. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Rat. Other species are not listed by the supplier.
Q.What immunogen was used to raise this antibody?
A.Recombinant rat Interferon gamma fragment (Gln23–Cys156), expressed in E. coli. Sequence identity with the human and mouse orthologs: 38% and 86%, respectively.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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