| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interferon gamma; IFN-gamma; IFNG |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived rat Interferon gamma recombinant protein (Position: Q23-C156). Rat Interferon gamma shares 38% and 86% amino acid (aa) sequence identity with human and mouse Interferon gamma, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of IFNG (Membrane cofactor protein) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Interferon gamma IFNG Antibody Picoband® (monoclonal, 8E9) catalog # M00393-2. Tested in ELISA applications. This antibody reacts with Rat.
Key elements and design rationale
- Antibody format: Mouse Monoclonal Mouse IgG1
- Clone number: Clone: Clone: 8E9
- Immunogen / epitope context: E. coli-derived rat Interferon gamma recombinant protein (Position: Q23-C156). Rat Interferon gamma shares 38% and 86% amino acid (aa) sequence identity with human and mouse Interferon gamma, respectively. (reported region: Q23-C156).
- Molecular weight context: reported MW: 53 kDa; calculated MW: nan
- Reactivity: Rat
- Applications: ELISA
As a monoclonal antibody, the reagent targets a defined epitope, supporting consistency across experiments; epitope masking by PTMs or conformational changes can affect signal.
Biological background
Membrane cofactor protein; interferon, gamma. Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation. Functional note: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Reported localization: Secreted. Expression/tissue context: Released primarily from activated T lymphocytes.
Research relevance and current trends
- Cancer: Researchers commonly examine how IFNG (Membrane cofactor protein) relates to this theme using model systems and orthogonal readouts.
- Cytokines: Researchers commonly examine how IFNG (Membrane cofactor protein) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how IFNG (Membrane cofactor protein) relates to this theme using model systems and orthogonal readouts.
Common research applications
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
