| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon gamma;IFN-gamma;Immune interferon;IFNG; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human IFN gamma. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes interferon gamma (gene IFNG) in human samples and is validated for Western blot. The predicted molecular weight is 19.3 kDa, and the supplier reports an observed band at about 19 kDa.
Interferon gamma is the only type II interferon. It is produced mainly by activated T cells and NK cells, signals through IFNGR1/IFNGR2 and the JAK1/JAK2–STAT1 pathway, and activates macrophages and antigen presentation, making it a key readout of Th1 and cytotoxic immune responses.
| Target | Interferon gamma (gene IFNG; UniProt P01579, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 19.3 kDa |
| Observed MW | 19 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): recombinant human IFN gamma protein.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the C-terminal region of human IFN gamma.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes interferon gamma as a secreted protein. Tissue expression noted by UniProt (human): Released primarily from activated T lymphocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Chunlin et al. (2018) Peptide Blocking of PD-1/PD-L1 Interaction for Cancer Immunotherapy. Cancer Immunology Research. 10.1158/2326-6066.CIR-17-0035
Cuijuan Liu et al. (2024) The peptide-based bispecific CAR T cells target EGFR and tumor stroma for effective cancer therapy. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2024.124558
