| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon gamma;IFN-gamma;Ifng; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived rat IFN gamma recombinant protein (Position: Q23-C156). Rat IFN gamma shares 38% and 86% amino acid (aa) sequences identity with human and mouse IFN gamma, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes interferon gamma (gene Ifng) in rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 17.9 kDa, and the supplier reports an observed band at about 19 kDa.
Interferon gamma is the only type II interferon. It is produced mainly by activated T cells and NK cells, signals through IFNGR1/IFNGR2 and the JAK1/JAK2–STAT1 pathway, and activates macrophages and antigen presentation, making it a key readout of Th1 and cytotoxic immune responses.
| Target | Interferon gamma (gene Ifng; UniProt P01581, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 17.9 kDa |
| Observed MW | 19 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| ELISA | 0.1–0.5 µg/mL |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Rat) |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant rat IFN gamma fragment (Gln23–Cys156), expressed in E. coli. Sequence identity with the human and mouse orthologs: 38% and 86%, respectively.
Reactivity Notes
The supplier lists reactivity with rat. UniProt describes interferon gamma as a secreted protein. Tissue expression noted by UniProt (rat): Released primarily from activated T lymphocytes.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Pengmin Chen et al. (2017) Tangshen Formula Attenuates Colonic Structure Remodeling in Type 2 Diabetic Rats. Evidence-based Complementary and Alternative Medicine. 10.1155/2017/4064156
