| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon gamma;IFN-gamma;Immune interferon;IFNG; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Interferon gamma |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOFD-9 is a rabbit monoclonal antibody that recognizes interferon gamma (gene IFNG) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 19.3 kDa, and the supplier reports an observed band at about 17 kDa.
Interferon gamma is the only type II interferon. It is produced mainly by activated T cells and NK cells, signals through IFNGR1/IFNGR2 and the JAK1/JAK2–STAT1 pathway, and activates macrophages and antigen presentation, making it a key readout of Th1 and cytotoxic immune responses.
| Target | Interferon gamma (gene IFNG; UniProt P01579, human) |
|---|---|
| Clone | AOFD-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 19.3 kDa |
| Observed MW | 17 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human interferon gamma.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes interferon gamma as a secreted protein. Tissue expression noted by UniProt (human): Released primarily from activated T lymphocytes.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shicong Wang et al. (2026) Pien-Tze-Huang inhibits colorectal cancer liver metastasis in mice by modulating immunosuppressive and fibrotic microenvironments. Translational Research. 10.1016/j.trsl.2026.06.014
Honglin Jia et al. (2022) Regulatory effects of miRNA-126 on Th cell differentiation and cytokine expression in allergic rhinitis. Cellular Signalling. 10.1016/j.cellsig.2022.110435
Yuke Li et al. (2024) Oridonin, a small molecule inhibitor of cancer stem cell with potent cytotoxicity and differentiation potential. European Journal of Pharmacology. 10.1016/j.ejphar.2024.176656
Xu Cong et al. (2025) Piceatannol improves autoimmune hepatitis by inhibiting the immune activities of T cells and macrophages through binding with c-Jun. Immunologic Research. 10.1007/s12026-025-09689-4
Yu-Ming Chen et al. (2015) Regional tissue immune responses after sciatic nerve injury in rats. International Journal of Clinical and Experimental Medicine. PMID: 26550275
