| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interferon gamma;IFN-gamma;Immune interferon;IFNG; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Interferon gamma |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-IFNG antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AOFD-9; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-Interferon gamma IFNG Rabbit Monoclonal Antibody catalog # M00393-1. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: IFNG (Interferon gamma).
- Antibody format: Monoclonal; clone AOFD-9; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
IFNG (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Reported cellular localization context: Secreted. Tissue expression notes (as provided): Released primarily from activated T lymphocytes.
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cytokines,Immunology,Innate Immunity,Interferons,Signaling Pathways,Stem Cells,TGF Beta,Tumor Immunology.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate IFNG antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect IFNG expression by Western blot in cell or tissue lysates, Localize IFNG by immunofluorescence/immunocytochemistry in cultured cells, Quantify IFNG-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 43 kDa; calculated MW: 19348 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 43 kDa
- Cellular localization (provided): Secreted.
- Tissue details (provided): Released primarily from activated T lymphocytes.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
