| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interferon regulatory factor 9;IRF-9;IFN-alpha-responsive transcription factor subunit;ISGF3 p48 subunit;Interferon-stimulated gene factor 3 gamma;ISGF-3 gamma;Transcriptional regulator ISGF3 subunit gamma;IRF9;ISGF3G; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human IRF9 recombinant protein (Position: M1-K110). Human IRF9 shares 87.3% amino acid (aa) sequence identity with mouse IRF9. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of IRF9 (Interferon regulatory factor 9) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Interferon regulatory factor 9/IRF9 Antibody Picoband® catalog # A04485. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human IRF9 recombinant protein (Position: M1-K110). Human IRF9 shares 87.3% amino acid (aa) sequence identity with mouse IRF9. (reported region: M1-K110).
- Molecular weight context: reported MW: 44 kDa; calculated MW: 43696 MW
- Reactivity: Human
- Applications: Flow Cytometry, IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Interferon regulatory factor 9; Interferon regulatory factor 9. Interferon regulatory factor 9 is a protein that in humans is encoded by the IRF9 gene, previously known as ISGF3G. It is mapped to 14q1. IRF9 is a 48 kDa member of the IRF family of proteins. It is widely expressed, and serves as a component of the ISGF3 complex. In addition, IRF9 is an interferon-dependent, positive-acting transcription factor that is cytoplasmically activated, possibly through interaction with the interferon receptor. It associates with activated STAT1 and STAT2 to form an ISGF3 complex that is translocated into the nucleus. Functional note: Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. Reported localization: Cytoplasm . Nucleus . Translocated into the nucleus upon activation by IFN-alpha/beta. Expression/tissue context: Leukocytes.
Research relevance and current trends
- Cell Adhesion: Researchers commonly examine how IRF9 (Interferon regulatory factor 9) relates to this theme using model systems and orthogonal readouts.
- Cell Type Markers: Researchers commonly examine how IRF9 (Interferon regulatory factor 9) relates to this theme using model systems and orthogonal readouts.
- Cytoskeleton/ECM: Researchers commonly examine how IRF9 (Interferon regulatory factor 9) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative IRF9 (Interferon regulatory factor 9) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of IRF9 (Interferon regulatory factor 9) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
