Anti-Interleukin-13 IL13 Antibody

SKU:BHA21000164
Research Validated
—
Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-IL13 antibody (Rabbit polyclonal, Rabbit IgG). Validated for ELISA. Reactivity: Rat. Recognizes interleukin-13. Supplied in lyophilized form, ready for use in standard immunodetection workflows. Optimize the working dilution for each application and sample type.
Target IL13
Host Rabbit
Reactivity Rat
Isotype Rabbit IgG
Application(s) ELISA
Options selector
Catalog no. Size Conjugation
RP1048 100 ug/vial
Available Options

Select the variant that best fits your experiment.

  • Options:
    • 100 ug/vial / Carrier Free, Unconjugated
      Form: Lyophilized
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
      Applications: ELISA (Cap)
      Application details: ELISA(Cap), 0.1-0.5μg/ml, Rat
      Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
    • 100 ug/vial / APC, Biotin, Cy3, FITC, Fluoro488, Fluoro550, Fluoro594, Fluoro647, PE
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Applications: WB,IHC,ELISA; Flow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cellsContents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 100 ug/vial / HRP
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: varies by selected option; see option details under Options.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target IL13
Alternative names Interleukin-13;IL-13;T-cell activation protein P600;Il13;Il-13;
UniProt # P42203
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Rat
Applications
  • ELISA
Immunogen E. coli-derived rat IL-13 recombinant protein (Position: P22-H131). Rat IL-13 shares 62% and 76% amino acid (aa) sequence identity with human and mouse IL-13, respectively.
Molecular weight 14.1 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Secreted.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) RP1048
Main SKU BHA21000164
Rabbit Polyclonal

Product Overview

This rabbit polyclonal antibody detects interleukin-13 (gene Il13) in rat samples and is validated for ELISA. The predicted molecular weight is 14.1 kDa.

Interleukin-13 (IL-13) is a type 2 cytokine produced by Th2 cells, group 2 innate lymphoid cells and mast cells. It signals mainly through the IL-4Rα/IL13RA1 receptor and STAT6, driving mucus production, airway hyperresponsiveness and tissue remodeling, and it is a central mediator in allergic inflammation, asthma and anti-parasite immunity.

Target Interleukin-13 (gene Il13; UniProt P42203, rat)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Rat
Form Lyophilized
Formulation Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers
Calculated MW 14.1 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

ELISA (capture antibody) 0.1–0.5 µg/mL (Rat)

Immunogen

Recombinant rat IL-13 fragment (Pro22–His131), expressed in E. coli. Sequence identity with the human and mouse orthologs: 62% and 76%, respectively.

Reactivity Notes

The supplier lists reactivity with rat. UniProt describes interleukin-13 as a secreted protein.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: ELISA (capture antibody) 0.1–0.5 µg/mL (Rat). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Rat. Other species are not listed by the supplier.
Q.Has this antibody been tested on primate samples?
A.Reactivity with primate samples has not been tested by the supplier. Aligning the immunogen sequence with the primate ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.Is a blocking reagent available for this antibody?
A.A matching blocking reagent for competition (specificity) controls can be supplied on request. Contact info@biohippo.com to request it.
Q.What immunogen was used to raise this antibody?
A.Recombinant rat IL-13 fragment (Pro22–His131), expressed in E. coli. Sequence identity with the human and mouse orthologs: 62% and 76%, respectively.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Jian Li et al. (2016) CD68 and interleukin 13, prospective immune markers for esophageal squamous cell carcinoma prognosis prediction. Oncotarget. 10.18632/oncotarget.6900

Bo Wang et al. (2015) Th17 Down-regulation Is Involved in Reduced Progression of Schistosomiasis Fibrosis in ICOSL KO Mice. PLoS Neglected Tropical Diseases. 10.1371/journal.pntd.0003434

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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