| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-6;IL-6;B-cell stimulatory factor 2;BSF-2;CTL differentiation factor;CDF;Hybridoma growth factor;Interferon beta-2;IFN-beta-2;IL6;IFNB2; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human IL-6 recombinant protein (Position: P29-M212). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes interleukin-6 (gene IL6) in human samples and is validated for Western blot. The predicted molecular weight is 23.7 kDa, and the supplier reports an observed band at about 20 kDa.
Interleukin-6 (IL-6) is a multifunctional cytokine released by macrophages, dendritic cells and many other cells during infection and tissue injury. It binds IL-6R and then the signaling subunit gp130, activating JAK–STAT3 through classic or trans-signaling. IL-6 drives the acute phase response, B-cell differentiation and Th17 development, and it is one of the most widely measured inflammatory markers.
| Target | Interleukin-6 (gene IL6; UniProt P05231, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 23.7 kDa |
| Observed MW | 20 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human IL-6 fragment (Pro29–Met212), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes interleukin-6 as a secreted protein. Tissue expression noted by UniProt (human): Produced by skeletal muscle. A carrier-free (BSA-free) format can be requested; specify it in the order note.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Shu Hai-Feng et al. (2010) Expression of the Interleukin 6 System in Cortical Lesions From Patients With Tuberous Sclerosis Complex and Focal Cortical Dysplasia Type IIb. Journal of Neuropathology and Experimental Neurology. 10.1097/NEN.0b013e3181eaeae5
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